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Caractérisation de bactéries pathogènes, Vibrio splendidus, isolées de bivalves marins ArchiMer
Gay, Melanie; Waechter, Magali; Lambert, Christophe; Escoubas, Jean-michel; Cochennec, Nathalie; Nicolas, Jean-louis; Berthe, Franck; Le Roux, Frédérique.
Nowadays, cupped oyster, Crassostrea gigas, production is the main shellfish production in France. Spats cultivated on most farms are usually collected in the wild, but hatchery spats are becoming more numerous, and represent around 10% of today's cultivated spats. During the summer period, when the temperatures are higher, high mortality rates have been regularly observed in cupped in oyster C. gigas (60 to 100%) spats since 1991, both in hatcheries-nurseries and in their natural habitat. Over the last five years, these episodes have become a big concern for all the professionals.
Tipo: Text Palavras-chave: Crassostrea gigas; Pathogène; Vibrio splendidus; Bactériologie.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3277.pdf
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Comparative analysis of Vibrio splendidus-related strains isolated during Crassostrea gigas mortality events ArchiMer
Le Roux, Frédérique; Gay, Melanie; Lambert, Christian; Waechter, Magali; Poubalanne, Saravanne; Chollet, Bruno; Nicolas, Jean-louis; Berthe, Franck.
French mollusc production is based mainly on the Pacific cupped oyster, Crassostrea gigas. Since 1991, annual mass mortality of juveniles has been reported during summer months. These recurring episodes concern professionals who fear that like Portugese oyster, C. angulata, C gigas could in turn disappear following one of these epizooties. Previously, bacteriological analysis of moribund oyster juveniles yielded an isolate of a Vibrio splendidus biovar H strain, named TNEMF6. This isolate was demonstrated to be pathogenic to Crassostrea gigas spat by experimental challenge. To study the association between summer oyster mortality and presence of TNEMF6 cluster strains, Vibrionaceae fauna were isolated from infected spat along the French Atlantic coast...
Tipo: Text Palavras-chave: Vibrio splendidus; Virulence; Epidemiology; PCR RFLP; Oyster mortality.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-565.pdf
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Recherche de bactéries pathogènes du genre Vibrio sur larves et naissain d'huître creuse, Crassostrea gigas, au sein d'une écloserie-nurserie de l'Ile de Ré ArchiMer
Waechter, Magali.
La production d'huître creuse, Crassostea gigas, est la principale production française de coquillages et la plus importante source de revenu du département de la Charente Maritime. Le naissain est principalement obtenu par captage naturel mais l'utilisation de naissain d'écloserie est en progression depuis quelques années. De forts taux de mortalité estivale, pouvant atteindre 60 à 100% sont rapportées actuellement dans les écloseries-nurseries, sur les larves et le naissain d'huître creuse, Crassostrea gigas. Dans le milieu naturel, la mortalité apparaît aussi sur le naissain de captage naturel. Cette mortalité est difficile à évaluer car les informations à ce sujet sont souvent contradictoires. En ce qui concerne le naissain de captage naturel, la...
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Ano: 1996 URL: http://archimer.ifremer.fr/doc/00428/53919/55041.pdf
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Recherche et identification de bactéries pathogènes au sein d'une écloserie nurserie d'huître creuse, Crassostrea gigas, en relation avec la mortalité estivale du naissain ArchiMer
Waechter, Magali.
Summer mortality has been observed on oyster spat Crassostrea gigas since 1991 on french coasts. In our study, we deseribed symptoms observed during the mortality, especially adductor muscle weakness and we isolated strains on Zobell medium and TCBS from moribund spat. Strains isolated during a high mortality (60 %) were selected by experimental pathology on spat. Inoculation by bath led us to select a pathogenic strain (TNEMF6) for oyster spat. Phenotypic identification, phylogenetic study on 16S rDNA and quantitative DNA/DNA hybridisation showed that this strain belongs to Vibrio splendidus biovar II. Phenotyping and ribotyping with strains isolated during the study showed that only TNNIII7 and TNMB5 are similar to the pathogenic strain TNEMF6....
Tipo: Text Palavras-chave: Mortalité estivale; Naissain; Crassostrea gigas; Ecloserie; Nursery; France; Bactériologie; Bactérie; Vibrio; Vibrio splendidus biovar II; Summer mortality; Oyster spat; Crassostrea gigas; Hatchery; Nursery; France; Bacteriology; Bacteria; Vibrio; Vibrio splendidus biovar II.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00211/32226/30682.pdf
Registros recuperados: 4
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